【摘要】 目的:了解脂联素基因SNP+45(T/G)单核苷酸多态性与哈尔滨地区汉族人2型糖尿病的关系。方法:自2004年黑龙江省哈尔滨地区的2型糖尿病流行病学调查资料中选出有糖尿病家族史,且相互间无亲缘关系的2型糖尿病患者180例,同时选择非糖尿病健康对照人群286例。所有研究对象均为汉族人,所有研究对象均签署了知情同意书。2型糖尿病诊断依据1999年WHO诊断标准:①有明确糖尿病病史。②空腹血浆葡萄糖≥7.0mmol/L和(或)口服糖耐量实验2h血糖≥11.1mmol/L。根据病史和家族史排除1型糖尿病、年轻起病的成人型糖尿病及线粒体性糖尿病。用聚合酶链反应-限制性片段长度多态(PCR-RFLP)方法鉴定脂联素基因SNP+45(T/G)单核苷酸多态性。结果:466例均进入结果分析。①2型糖尿病组中TT、TG、GG基因型分别为89例(48.4%)、79例(42.9%)、12例(6.67%),正常对照组中TT、TG、GG基因型分别为152例(53.1%)、114例(39.9%)、20例(6.99%)。SNP+45(T/G)多态位点的基因型频率和等位基因频率在2型糖尿病组和正常对照组中的分布差异无显著性(P>0.05)。②分别以腰围(男性≥90cm,女性≥80cm为肥胖)和体质量指数(体质量指数≥28kg/m2为肥胖;24kg/m2≤体质量指数<28kg/m2为超重)判定超重、肥胖,SNP+45(T/G)多态位点的基因型频率和等位基因频率在各组别中的分布差异无显著性(P>0.05)。③男性和女性分群体分析,在同一性别群体中,SNP+45(T/G)多态位点的基因型频率和等位基因频率在2型糖尿病组和正常对照组中的分布差异无显著性(P>0.05);SNP+45(T/G)的基因型频率和等位基因频率在男性和女性间分布差异也无显著性(P>0.05)。结论:脂联素基因SNP+45(T/G)单核苷酸多态性位点与哈尔滨地区汉族人2型糖尿病无明显相关性。更多还原

【Abstract】 AIM: To investigate the association between the polymorphism of SNP+45(T/G) in adiponectin gene and type 2 diabetes in Harbin Han population. METHODS: 180 type 2 diabetes patients with family history in the epidemiological study data of type 2 diabetes in Harbin, Heilongjiang province in 2004, who had no relationship with each other, and 286 non-diabetes subjects were selected as control. All the subjects with type 2 diabetes had the family history of type 2 diabetes. All the subjects were Han people and signed the informed consent before participation. Type 2 diabetes was diagnosed in accordance with the diagnostic criteria of World Health Organization (1999): ①Subjects with past history of type 2 diabetes; ②Fasting plasma glucose (FPG)≥ 7.0 mmol/L and (or) oral glucose tolerance test (OGTT) 2-hour plasma glucose ≥ 11.1 mmol/L. According to the case history and family history of diabetes, subjects with type 1 diabetes, maturity-onset diabetes of youth and mitochondria gene defects diabetes were excluded. PCR-RFLP method was used to determine the polymorphism of the SNP+45(T/G) in exon 2 of adiponectin gene. RESULTS: 466 subjects were all involved in the results analysis. ① In the type 2 diabetes group, the TT, TG and GG genotype were found in 89 cases (48.4%), 79 (42.9%) and 12 (6.67%), respectively, and in the control group were found in 152 (53.1%), 114 (39.9%) and 20 (6.99%) cases, respectively. There was no significant difference in the distribution of genotype and allele frequency between the two groups (P > 0.05). ②Overweight and obesity were determined separately according to the waist circumference (male ≥ 90 cm, female ≥ 80 cm as obesity) and body mass index (BMI ≥ 28 kg/m2 as obesity; 24 kg/m2 ≤ BMI < 28 kg/m2 as overweight), and the distribution of the genotype and allele of SNP+45(T/G) had no significant difference between the two groups (P > 0.05). ③In the same sex group, there was no gender difference in the distribution of the genotype and allele between the two groups (P > 0.05), and there was also no significant difference between the subjects with type 2 diabetes and the control both in men and women group (P > 0.05). CONCLUSION: There is no obvious association between the polymorphism sites of SNP+45(T/G) in adiponectin gene and type 2 diabetes in Han population of Harbin.更多还原