【摘要】 目的:观察人工关节磨损产物金属离子钴(Co2+)、铬(Cr3+)对人工关节无菌性松动的影响。方法:试验于2005-06/2006-01在南昌大学第一附属医院烧伤实验室完成。①将CoCl2粉末与CrCl3粉末溶于无菌注射用水配制成CoCl2溶液和CrCl3溶液,使用前内毒素鳌试剂检测均证实无内毒素。②用lymphoflot和Percoll梯度离心法获得志愿者外周血中的单核细胞,将细胞在24孔培养皿中培养,4×106个/孔。③将CoCl2溶液和CrCl3溶液分别加入人单核细胞液中,使其终末溶度分别为1×10-5和5×10-4,单核细胞单独培养作为对照组。④分别于12,24,48h取细胞上清液,用ELISA法检测上清液中肿瘤坏死因子含量,以吸光度(A值)的高低来反映标本中肿瘤坏死因子α含量的多少。结果:①Co2+刺激单核细胞分泌肿瘤坏死因子结果:Co2+与人单核细胞共培养后,12,24,48h检测上清液A值分别为0.6362±0.2993,1.0915±0.4081,0.7150±0.2951,实验组24h肿瘤坏死因子含量明显高于12h和48h肿瘤坏死因子含量。②Cr3+刺激单核细胞分泌肿瘤坏死因子结果:Cr3+与人单核细胞共培养后,12,24,48h上清液A值分别为0.4937±0.1165,0.8727±0.2801,0.5088±0.2310,与Co2+类似,与Cr3+共培养后单核细胞上清液A值在24h达到峰值,48h又下降。结论:金属离子Co2+,Cr3+能通过刺激人单核细胞释放肿瘤坏死因子,诱发假体周围骨溶解,进而引起假体松动。更多还原

【Abstract】 AIM:To discuss the effect of artificial joint wear production meal ion cobalt(Co2+) and chromium(Cr3+) on aseptic loosening of artificial joint.METHODS:The experiment was accomplished from June 2005 to January 2006 in the Burn Laboratory of the First Hospital Affiliated to Nanchang University.①CoCl2 powder and CrCl3 powder were dissolved in asepsis injection water for preparing the corresponding solutions,and confirmed with endotoxin before use.②Lymphofolt and Percoll gradient centrifugation were used to acquire mononuclear cell from peripheral blood,then the cells were cultured in the 24-hole plate,with 4×106 cell per hole.③CoCl2 solution and CrCl3 solution were added into monocyte liquid to adjust their final concentration to 1×10-5 and 5×10-4,while mononuclear cells cultured alone were served as negative.④After cells were incubated for 12,24 and 48 hours,ELISA method was applied to detect tumor necrosis factor(TNF) α content in serum supernatant via the absorbance ?穴A?雪.RESULTS:①When monocytes were exposed to Co2+ for 12,24 and 48 hours,A value of serum supernatant was 0.636 2±0.299 3,1.091 5±0.408 1,0.715 0±0.295 1.TNF α content was obviously higher at hour 24 than at hours 12 and 48.②When monocytes were co-cultured with Cr3+,A value arrived the peak at hour 24 and declined at hour 48(0.493 7±0.116 5,0.872 7±0.280 1,0.508 8±0.231 0 at hours 12,24 and 48).CONCLUSION:Co2+ and Cr3+ can stimulate human mononuclear cells release TNF α and induce osteolysis,resulting in aseptic loosening of artificial joint.更多还原