【摘要】 探讨伏马菌素B1(FB1)对体外培养的人外周血单个核细胞(hPBMC)抗原加工相关转运子(TAP-1)表达的影响。采用流式细胞定量检测(FCM)、免疫印迹(Western印迹)及半定量RT-PCR方法,研究不同浓度FB1(0,10和50μmol/L)处理后人外周血单个核细胞TAP-1在mRNA和蛋白质水平表达的影响。RT-PCR检测结果表明,10和50μmol/L FB1处理24h后,处理组细胞TAP-1mRNA明显低于对照组。在蛋白质水平,FCM定量分析表明,两个处理组细胞表面TAP1的平均荧光强度均较对照组降低,以50μmol/LFB1处理组降低显著(P<0.05)。免疫印迹结果亦表明,FB1处理组TAP-1的表达均较对照组降低。10和50μmol/LFB1可抑制体外培养的hPBMCTAP-1mRNA和蛋白质表达。更多还原

【Abstract】 To explore the effects of fumonisin (FB1) on TAP-1 (transporter associated with antigen processing) expression of human peripheral blood mononuclear cells (hPBMC) in vitro. The expression of TAP-1 of hPBMC by FB1 pretreatment at three different dosages (0,10 and 50 μmol/L) were detected with flow cytometry (FCM),Western blot and semi-RT-PCR respectively. TAP-1 mRNA was decreased in 10 and 50 μmol/L FB1 treated groups than that in control group. FCM quantitative analysis showed that at protein level,TAP-1 expression (as expressed by FI) of hPBMC at FB1 treated groups were lower than that of control,especially in 50 μmol/L FB1 treated groups (P<0.05). Western blot results further confirmed the above results. 10 and 50 μmol/L FB1 treatment could inhibit the expression of TAP-1 at mRNA and protein level.更多还原