【摘要】 应用直接注射法制作ICR小鼠原发肝癌模型并成功地进行方法改进。对模型小鼠进行了体重监测、原位移植成功率统计和病理检查,结果表明改进后的直接注射法制作小鼠原发肝癌模型的成功率显著提高,并成功模拟出肿瘤的多器官转移及人类肝癌常见的临床症状、体征。模型可以很好的满足抗肿瘤药物开发、肝癌发生发展机制研究的需要。同时,直接注射法改进方案和理念解决了直接注入细胞产生返漏的技术难题,为进一步建立其他脏器的原发或继发性癌症移植模型打下了基础。更多还原

【Abstract】 Primary hepatocarcinoma ICR mouse model was successfully established with improved injection method. The model mice were analyzed by weight survey, success ratio of primary transplant and patho-logical analysis. Results demonstrated that the improved method increased the success ratio greatly, simulated multiple-organ metastasis and common symptoms of human liver cancer well. The model can satisfy the needs from exploitations of anti-cancer drugs and researches on cancer mechanism. In addition, the protocol and idea in the method not only solved the technical difficulty of injection method caused by tumor cells leaking but also laid the foundation for establishment of other primary and secondary cancer models.更多还原