【摘要】 为了研究山羊FSHR基因对产羔性状的影响,用RFLP-PCR法对多胎和单胎布尔山羊、关中奶山羊和萨能奶山羊母羊FSHR基因5′端侧翼序列进行了多态性检测。结果发现,FSHR基因5′端侧翼序列经PstⅠ、HaeⅢ、SinⅠ和TaqⅠ酶切,在多胎母羊和单胎母羊群体中均未表现出多态性;不同山羊群体FSHR基因5′端侧翼序列中各存在一些碱基的差异,但这些差异均未影响FSHR基因的转录和山羊的产羔性状。说明所检测的FSHR基因5′端侧翼序列这些酶切位点与山羊的产羔性状之间没有遗传连锁关系。更多还原

【Abstract】 Boer goat,Guanzhong goat and Xinongsaneng goat with multiplets and monovular traits were used to study the effect of follile-stimulating hormone receptor gene on yeant rait.Polymorphism of the 5′-flanking region of goat follile-stimulating hormone receptor gene was analyzed by PCR-RFLP as the candidate gene.The result showed there wasn’t polymorphism in goat.Difference of several bases were found by sequening,but the transcription of follile-stimulating hormone receptor gene and yean trait of goat were not affected,which showed that there was not the genetic linkage relationship between the four sites of 5′-flanking region of goat FSHR gene and its yean trait.更多还原