In order to distinguish the species of root-knot nematode occurring in greenhouse in Shaanxi Province,and provide effective prevention,seventy-one root-knot nematode samples were collected from Shaanxi Province.Based on morphology and mtDNA,M.incognita,M.javanica and M.arenaria were identified.Results showed M.incognita was the dominant specie in greenhouse in Shaanxi.So,M.incognita should be the main control object,at the same time,the mixed infection should be noticed,too.