【摘要】 金黄壳囊孢菌(Cytospora chrysosperma)是一类重要的树木腐烂病菌,其培养性状和真菌菌丝的营养体亲和性能够反映真菌的遗传、形态、生理、病理和生态等方面的差异。为了探讨金黄壳囊孢菌的遗传变异和遗传多样性,研究对采自我国9个省份的12个杨树品种上的30株C.chrysosperma在4种培养基中的培养性状进行了观察,并在PDA培养基上进行了真菌的营养体亲和性试验。结果表明,供试金黄壳囊孢菌在培养性状上存在明显的种群分化,通过模糊聚类可将30个菌株划分为8个组,且其亲缘关系的远近与地理来源有明显的相关性;30个菌株在PDA上的亲和性配对试验结果表明,绝大多数菌株间产生黑色色素带,少数菌株以及同一菌株不同菌落间不产生此色素带,30个菌株归为25个营养体亲和群(VCG),其中21个VCG各只含1个菌株,1个VCG含3个菌株,只有3个VCG各含2个菌株。研究结果说明供试菌株的营养体亲和性与其地理来源无明显相关性。更多还原

【Abstract】 Cytospora chrysosperma is a major pathogenic fungi of trees rot diseases and its cultural morphology and vegetative compatibility could reflect the differentiation of genetic in morphology,physiology,and pathogenic and geographical region.In order to investigate the genetic change and diversity of Cytospora chrysosperma,thirty isolates of C.chrysosperma were obtained on 12 poplars from 9 provinces in china,and their different cultural morphology on four culture mediums and vegetative compatibility on PDA were observed.The results showed that there were significant cultural and morphological differences among isolates of C.chrysosperma.Based on cultural morphology the isolates could be divided into 8 groups and had relation with geographical position.Most of isolates produced incompatible reaction by mycelial parings on PDA in which a reaction color line(dark green) between the two isolates developed in interaction zone;few isolates and same isolates were compatible reaction in which no reaction color line developed.The 30 isolates were divided into 25 vegetative compatibility groups(VCGs).One of those 25 VCGs included 3 isolates,three of those 25 VCGs included 2 isolates,and 21 only included 1 isolates respectively.This study showed no apparent correlation between the vegetative compatibility groups and the geographical resource region of the fungal isolates.更多还原