【摘要】 应用甜瓜离体叶片检测甜瓜种子的带菌情况,分别采用不同的缓冲液和不同的接菌方法进行检测,发现PBS缓冲液富集病原菌效果好,注射接种效率高。同时,离体叶片是菌株致病力分化初步检测较好的材料,从田间分离所得菌株来看,黄瓜细菌性角斑和西瓜果腐菌株致病力均被分化为强、中、弱,而且从种子上分离的病原菌的致病力弱。更多还原

【Abstract】 The different buffer solution separately examination the ratio of melon seed′s disease-carrying using leaf in vitro was conducted.The result showed that PBS buffer solution which had better effect to enrich bacteria.And the injection vaccination efficiency is high.The leaf is better material in vitro for pathogenic differentiation.Pseuodomonas syringae pv.Lachryman and Acidovorax avenae subsp.citrulare are compartmentalized as strong,middle and weak in pathogenic differentiation,disease germ′s pathogenesis strength is weak separated from the seed.更多还原