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利用SDS-PAGE分析我国小麦品种的HMW-GS组成与分布

Study the Composition and Distribution of the HMW-GS of the Wheat Varieties in China by SDS-PAGE

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【作者】 许琦; 王彩萍; 刘春雷; 徐杰; 黄峰; 尤明山; 李保云; 刘广田;

【Author】 XU Qi1,2,WANG Cai-ping1,LIU Chun-lei1,XU Jie1,HUANG Feng1,YOU Ming-shan1,LI Bao-yun1* and LIU Guang-tian1(1.Beijing Key Laboratory of Crop Genetic Improvement,Key Laboratory of Crop Genomics & Genetic Improvement,Ministry of Agriculture,the People’s Republic of China,China Agricultural University(CAU),Beijing100094,China;2.Cotton Research Institute of Shanxi Academy of Agricultural Sciences,Yucheng Shanxi 044000,China)

【机构】 中国农业大学/农业部作物基因组学与遗传改良重点开放实验室/北京市作物遗传改良重点实验室; 中国农业大学/农业部作物基因组学与遗传改良重点开放实验室/北京市作物遗传改良重点实验室 北京100094; 山西省农科院棉花研究所; 山西运城044000; 北京100094;

【摘要】 以我国自育的250个小麦品种(系)为材料,分析了我国小麦品种HMW-GS的组成和分布。结果显示,我国小麦品种(系)的HMW-GS变异类型非常丰富,总共发现了19种变异类型,其中包括4种稀有变异类型;从遗传变异指数可以看出,HMW-GS等位基因遗传多样性Glu-B1>Glu-A1>Glu-D1;从亚基的组成和分布来看,我国小麦品种中优质亚基的比例是比较低的,在Glu-A1位点,2*亚基的比例为15.2%,在Glu-B1位点,14+15亚基的比例为12.4%,17+18亚基的比例为2.4%,在Glu-D1位点,5+10亚基的比例为23.6%。

【Abstract】 In this study,250 Chinese wheat varieties(lines) were used to analyse the diversity of HMW-GS.The results were as follows: There was a great diversity of HMW-GS among Chinese wheat,19 HMW-GS variation types were found including 4 unusual types.By comparing the diversity of the alleles of HMW-GS according to the index of genetic variability,the order could be got as Glu-B1>Glu-A1>Glu-D1.In addition,from the components and distribution of the HMW-GS,it could be concluded that the proportion of the subunit with good bread-making such as 2*,5+10,14+15 and 17+18 was relatively lower,the percents were 15.2%,12.4%,2.4%and 23.6% respectively.

【关键词】 小麦; HMW-GS; 遗传组成;
【Key words】 Wheat; HMW-GS; Genetic composition;
【基金】 国家自然科学基金(30270823,30471076);国家高技术研究发展计划“863计划”(2002AA207003,2003AA207080)资助
  • 【文献出处】 西北农业学报 ,Acta Agriculturae Boreali-Occidentalis Sinica , 编辑部邮箱 ,2007年01期
  • 【分类号】S512.1
  • 【被引频次】4
  • 【下载频次】193
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