【摘要】 目的:初步确定健康人外周血中具有CD4+CD25nt/hiCD127lo特征的调节性T细胞(Treg)频率,为临床相关疾病的研究及Treg的分选提供参考。方法:采集312名8~60岁(5个年龄组)、不同性别健康人的静脉血,经三重免疫荧光染色,用流式细胞术分析CD4+CD25nt/hiCD127loTreg细胞频率,并观察细胞内Foxp3转录因子的表达。结果:健康人CD4+CD25nt/hiCD127loTreg细胞在外周血中约占CD4+T细胞的(6.55±0.11)%,各年龄组之间有差异(P=0.015),组内性别之间也存在统计学意义(P<0.05);CD25nt/hiCD127lo细胞特异性地表达Foxp3转录因子。结论:初步确定了中国健康人外周血中具有CD4+CD25nt/hiCD127lo表达特征的细胞频率,为Treg细胞的临床研究奠定了基础;CD25nt/hiCD127lo作为CD4+CD25+Treg细胞表面的特征性标志,可在分选时排除其他细胞干扰,获得较完整的Treg细胞。更多还原

【Abstract】 AIM: To determine the frequency of the CD4+CD25nt/hiCD127lo regulatory T lymphocytes from the peripheral blood in the Chinese healthy individuals and provide some useful evidence for clinical research of correlative diseases.METHODS: From the CD4+CD25nt/hiCD127lo regulatory T lymphocytes of peripheral blood in 312 Chinese healthy male and female individuals aged from 8 to 60(five age groups were collected) The expression of transcription factor Foxp3 was detected by triplex immuno fluorescence and the frequency of CD4+CD25nt/hiCD127lo regulatory T lymphocytes was determined by flow cytometry.RESULTS: The frequency of CD4+CD25nt/hiCD127lo regulatory T lymphocytes in Chinese healthy individuals was(6.55±0.11)%,and the frequency differed among age groups(P=0.015) and sex groups(P<0.05).CD4+CD25nt/hiCD127lo regulatory T lymphocytes specifically express transcription factor Foxp3.CONCLUSION: The frequency of the CD4+CD25nt/hiCD127lo regulatory T lymphocytes from the peripheral blood in the Chinese healthy individuals has benn preliminarily determined which lays the foundation for further clinical research of regulatory T lymphocytes.As a specific cell surface marker,CD25nt/hiCD127lo can helpful obtain pure CD4+CD25+ regulatory T lymphocytes and suppress the interference of other cells duringcell separation.更多还原