【摘要】 目的:探讨伏马菌素B1(FB1)对体外培养的人外周血单个核细胞表面(HPBMc)HLA-Ⅰ分子表达的影响。方法:采用流式细胞术(FCM)、Western blot及半定量RT-PCR方法,研究不同浓度FB1(10和50μmol/L)处理后人外周血单个核细胞表面HLA-Ⅰ分子表达的变化。结果:FCM定量分析结果表明,经FB1处理24 h后,两组FB1处理细胞HLAⅠ-的平均荧光强度均较对照组降低(P<0.05),但是在两个处理组之间无统计学意义。Western blot也证实了上述结果。在mRNA水平上,分别检测了HLA-Ⅰ分子3个等位基因HLA-A,HLA-B,HLA-C的表达情况。结果显示,FB1处理后,HLA-A、HLA-B mRNA的表达均没有明显影响,仅HLA-C mRNA的表达较对照组降低。结论:10和50μmol/L FB1处理24 h可抑制人外周血单个核细胞表面HLA-Ⅰ分子的表达。更多还原

【Abstract】 AIM: To study the effects of Fumonisin B1(FB1)on HLA-Ⅰon human peripheral blood mononuclear cells(PBMC) in vitro.METHODS: The expression of HLA-Ⅰ on PBMC by FB1 pretreatment at different dosage(10,50 μmol/L)was detected with flow cytometry(FCM),Western blot,and semi-RT-PCR,respectively.RESULTS: The expression of HLA-Ⅰ on PBMC in vitro at the two experimental concentration was both lower than that of the control after FB1 treatment for 24 h as represented by fluorescence intensity by FCM analysis.Western blot results further confirmed the above results.At mRNA level,HLA-A,B and C mRNA were detected by RT-PCR,and the results showed that no changes were found on the expression of HLA-A,B mRNA between FB1 treated group and the control group,but HLA-C mRNA was inhibited in FB1 treated groups.CONCLUSION: 10 and 50 μmol/L FB1 could inhibit the expression of HLA-Ⅰon human PBMC in vitro at 24 h treatment.更多还原