【摘要】 目的探索合成Aβ_1-15多重抗原肽疫苗的方法,并对其免疫学活性进行鉴定。方法采用间接偶联法合成8分支Aβ_1-15多重抗原肽（MAP）,通过高效反相液相色谱（RP-HPLC）、SDS-PAGE以及氨基酸成分分析对其进行初步鉴定。以合成的MAPAβ_1-15免疫C57BL/6小鼠,用ELESA法检测血清特异性抗Aβ抗体。结果间接偶联法合成的MAPAβ_1-15,经RP-HPLC层析后,呈现为一宽大主峰。SDS-PAGE检测显示,共有8条蛋白带,条带梯度比较均匀,分别为1⁸分支的MAPAβ_1-15,氨基酸组份及含量基本与Aβ_1-15多肽的序列一致。以合成的MAPAβ_1-15疫苗免疫C57BL/6小鼠后,可产生高滴度的特异性抗Aβ抗体。结论间接偶联法可成功地合成MAPAβ_1-15疫苗,且具有很好的免疫学活性,但合成的MAP产物难以纯化。更多还原

【Abstract】 AIM: To explore the method of synthesizing the Aβ_1-15 multiple antigen peptide（MAP）vaccine and to identify its quality and the immunological activity. METHODS: MAP Aβ_1-15 was synthesized by indirect conjugation and analyzed by RP-HPLC, SDA-PAGE and amino acid analysis. Then, C57BL/6 mice were immunized with synthesized MAP Aβ_1-15. The specific anti-Aβ antibody in the sera of the immunized mice was identified by ELSA. RESULTS: There was a high and wide peak wave in the RP-HPLC chromatogram. The 8 protein bands identified by SDA-PAGE was identical with 1 to 8 branch of MAP Aβ_1-15. The amino acid sequence of synthesized MAP Aβ_1-15 was almost similar with the standard. High titer of anti Aβ antibody was obtained in the C57BL/6 mice immunized with MAP Aβ_1-15. CONCLUSION: MAP Aβ_1-15 could be synthesized successfully by indirect conjugation and the synthesized MAP Aβ_1-15 had satisfactory immune activity. But the purification of the synthesized complex remained to be a problem.更多还原