【摘要】 目的通过检测原发性干燥综合症(pSS)患者血清白细胞介素-21(IL-21)的表达水平,探讨其水平变化与pSS患者其他实验室检测指标关系以及其在pSS发病中的作用。方法选取符合2002年修订的pSS国际分类标准的40例初诊pSS患者。采用双抗体夹心ELISA方法检测该组患者及30例正常对照者血清中IL-21的水平。同时,采用免疫化学发光法检测患者的甲状腺情况包括FT3、FT4、TSH、TgAb、TPOAb。抗SSA和抗SSB抗体、ESR、血清蛋白电泳检测分别采用免疫双扩散法、魏氏法、全自凝胶法。并与患者的其他临床表现进行统计学分析。结果pSS患者血清IL-21水平为(1051±335)ng/L,明显高于正常对照组水平[(466±90)ng/L,P<0.05]。特别是pSS患者中抗体阳性组、有腮腺肿痛组、皮疹组、合并甲状腺功能减退组血清IL-21水平均高于各自阴性对照组,差异具有统计学意义(P<0.05)。而且pSS患者中IL-21水平与γ-球蛋白及血沉存在明显正相关(r=0.715,P<0.05;r=0.740,P<0.05)。结论pSS患者血清IL-21水平明显升高,且与γ-球蛋白及血沉存在明显正相关,提示IL-21可能参与pSS的发病过程。更多还原

【Abstract】 AIM: To investigate the relation between the change of serum IL-21 level and other laboratory indexes by detecting serum IL-21 level from patients with primary Sjgren’s syndrome (pSS) and explore the role of IL-21 in pSS. METHODS: The level of serum IL-21 from 40 pSS patients diagnosed according to 2002 revised international classification criteria for pSS and 30 healthy persons were detected by enzyme-linked immunosorbent assay(ELISA) respectively . Meanwhile, FT3, FT4, TSH, TgAb, TPOAb were detected by Immunochemiluminescent technique. Double diffusion method, Westergren and automatic gel method detected ant-SSA and ant-SSB antibody, ESR and serum protein electrophoresis respectively. The relationship between serum IL-21 level and other clinical symptom of the patients was analyzed. RESULTS: The level of serum IL-21 in patients with pSS (1051±335) ng/L was obviously higher than that in healthy control (466±90 ng/L), (P<0.05). The levels of serum IL-21 in the self-antibody positive group, parotid gland swelling pain group, rash group and incorporating hypothyroidism group were higher than those in their negative control groups, and there were significant differences(P<0.05). In addition, The level of serum IL-21 level in pSS patients showed positive correlation with γ-globulin (r=0.719, P<0.05) and ESR level(r=0.745, P<0.05). CONCLUSION: The evident increase of serum IL-21 level in pSS patients has positive correlation with the levels of γ-globulin and ESR, which suggests that IL-21 may play a critical role in the pathogenesis of pSS.更多还原