【摘要】 目的研究川芎嗪(tetramethylpyrazine,TMP)对大鼠脑缺血再灌注损伤后海马齿状回(dentate gyrus,DG)细胞增殖的影响。方法成年雄性SD大鼠行2 h大脑中动脉阻塞手术,术后2 h开始腹腔注射TMP[40 mg/(kg.d)]。手术后腹腔注射5-溴脱氧尿核苷(5-bromodeoxyuridine,BrdU),末次注射24 h后处死动物,免疫组化染色观察TMP对脑缺血再灌注损伤后DG细胞增殖的作用。结果正常组和假手术组在DG有少量BrdU阳性细胞,对照组缺血后1 d阳性细胞开始增加,14 d达到高峰(P<0.05),TMP治疗组缺血后1 d损伤侧BrdU阳性细胞数开始增加,7 d达高峰(P<0.05)。结论TMP能促进缺血再灌注损伤大鼠海马齿状回内源性神经干细胞增殖。更多还原

【Abstract】 Objective To observe the effects of tetramethylpyrazine(TMP) on cell proliferation in dentate gyrus(DG) in rat brain after cerebral ischemia-reperfusion injury.Methods Male SD rats were randomly divided into normal,sham-operation,control and TMP treatment groups.The control and TMP treatment groups were further divided into 5 subgroups: 1 d,3 d,7 d,14 d and 21 d reperfusion after 2 h middle cerebral artery occlusion(MCAO).The focal cerebral ischemia-reperfusion model was made by MCAO.S phase cells were labelled with BrdU.Immunohistochemistry method was conducted to detect the BrdU positive cells.The total number of BrdU positive cells in the DG was measured.Results There was a significant increase of BrdU positive cells in DG of TMP treatment on 3 d and 7 d groups compared to that in control group(P<0.05).The total number of BrdU positive cells reached a peak on d 7 and declined afterwards.Conclusion Tetramethylpyrazine may promote cell proliferation in DG in adult rats after ischemia-reperfusion injury.更多还原