【摘要】 目的:探讨环氧化酶-2选择性抑制剂nimesulide对乳腺癌细胞株MMP-2、MMP-9mRNA表达的影响。方法:应用逆转录聚合酶链反应(RT-PCR)的方法,检测不同浓度nimesulide作用体外培养的MDA-MB-231、MCF-7人乳腺癌细胞株48h,MMP-2、MMP-9mRNA的变化。结果:50 μ mol/L nimesulide明显抑制MDA-MB-231细胞MMP-2mRNA的表达,100 μ mol/L时其几乎完全抑制;100 μ mol/L nimesulide显著抑制MCF-7细胞MMP-2mRNA的表达,200 μ mol/L时完全抑制其基因的表达。50 μ mol/L nimesulide明显抑制MDA-MB-231、MCF-7细胞MMP-9mRNA的表达,随着浓度的增加,MMP-9mRNA逐渐被抑制。结论:nimesulide以剂量依赖性方式下调MDA-MB-231、MCF-7乳腺癌细胞株MMP-2、MMP-9mRNA的表达,而MDA-MB-231细胞对nimesulide更敏感,这种作用是COX-2非依赖性途径,为nimesulide用于转移乳腺癌的治疗与预防提供了实验依据。更多还原

【Abstract】 Objective:To investigate the effect of nimesulide of the expressions of MMP-2、MMP-9 mRNA in human breast cancer cell lines,including MDA-MB-231 (overexpression COX-2) and MCF-7 (no expression of COX-2). Method: RT-PCR was used to determine the levels of expressions of MM-2、MMP-9 mRNA in MDA-MB-231,MCF-7 cells treated at different concentrations of nimesulide for 48h,respectively. Results: Nimesulide significantly suppressed MMP-2,MMP-9 mRNA expressions in MDA-MB-231 cell line at 50μmol/L nimesulide,and in MCF-7 cell line at 100～200μmol/L,in a does-dependent manner. Conclusions: Nimesulide could down-regulate the amount of MMP-2,MMP-9 mRNA expressions in a does-dependent manner. The expressions of these genes were more sensitive to nimesulide in MDA-MB-231 than that in MCF-7 cell line. The mechanism,which wasn’t dependent on COX-2 expression,may provide the experimental evidence for nimesulide used for the prevention and treatment of the metastatic breast cancer.更多还原