【摘要】 介绍一种提取苔藓植物毛尖紫萼藓(Grimmia pilifera P.Beauv)总RNA的方法。以新鲜的紫萼藓为材料,采用改良的SDS的方法,以氯化锂为沉淀试剂,成功地提取了该植物的总RNA。并与其他方法作了对比,结果发现该方法获得的RNA条带清晰、完整性好、纯度高、DNA污染小。可满足反转录及RT-PCR等实验要求。更多还原

【Abstract】 A method of extracting total RNA from Grimmia pilifera P.Beauv of moss was introducted.The fresh Grimmia pilifera was used as the main materials.The improved SDS method was implied,and the reagent of RNA precipitation was LiCl.The total RNA were successfully isolated from this plant.At the same time,the method was compared with others,it could be find that the total RNA of G.pilifera extracted by the method had clear bands,good completeness,high purity and less DNA contamination.The total RNA is suitable for Reverse Transcription,RT-PCR.更多还原