【摘要】 研究了脂肪酶固定化及其催化大豆油制备生物柴油的工艺。采用溶胶-凝胶法对脂肪酶进行了固定化,考察了固定化酶催化大豆油转酯化的生产工艺中酶用量、醇油比、含水量、反应温度、反应时间、溶剂等参数对转酯过程的影响。实验结果表明,当大豆油4.5 g时,最佳的反应条件为:固定化酶646 mg,醇油摩尔比4∶1,含水质量分数为6%,40℃,甲酯的最终转化率为96.33%。更多还原

【Abstract】 In this manuscript lipase from Pseudomonas cepacia was immobilized in sol-gel matrix and use to its transesterification of soybean oil with methanol into biodiesel was studied.The effects of water content, methanol/oil molar ratio,enzyme loading,temperature,organic solvents and time course on the transesterification were determined.The results showed that the optimal conditions for transesterification were as follows: soybean oil 4.5 g: temperature 40 ℃,4∶1 methanol/oil molar ratio,6% water content and 646mg immobilized lipase.By combination with the optimum conditions,a high methyl esters formation(96.33%) was obtained.更多还原