【摘要】 从广西北海、桂林地区11个柑橘品种溃疡病材料上分离得到13株致病菌,进行菌系分化研究表明:在所测试的35项生理特征中有25项(柠檬酸盐、丙二酸盐、36℃生长、耐盐性(1～3%)、吐温20、吐温80、水杨素、卫茅醇、葡萄糖、乳糖、纤维二糖、苯丙氨酸、棉子糖、松三糖、侧金盏花醇、菊糖、核糖、甘露醇、阿拉伯糖、海藻糖、蜜二糖、山梨糖、七叶苷、酒石酸盐、明胶)完全相同,10项(醋酸盐、果糖、枸橼酸盐、鼠李糖、木糖、蔗糖、麦芽糖、半乳糖、淀粉、甘露糖)存在差异;16项生化特征测试中有12项(细胞色素C氧化酶、过氧化氢酶、甲基红试验、乙酰甲基甲醇试验、水解七叶苷、果聚糖产生、硝酸盐还原、脲酶、苯丙氨酸脱氨酶、精氨酸双水解酶、卵磷脂酶、明胶水解)完全相同,4项(水解淀粉、吲哚产生、硫化氢产生、石蕊牛奶)存在差异;3种噬菌体鉴定试验能够区分出13个菌株菌系分化现象的存在,且分为6种敏感型,而菌株细胞脂肪酸成分分析则不能区分菌系分化情况。更多还原

【Abstract】 Thirteen strains isolated from diseased materials of 11 citrus varieties collected from Beihai and Guilin districts were studied.Among 35 physiological characteristics assayed in 13 strains,25 items including citrate,malonate,growth at 36℃,NaCl tolerance,Tween 20,Tween 80,salicin,dulcitol,glucose,lactose,cellubiose,phenylalanine,raffinose,melezitose,adonitol,synanthrin,ribose,mannitol,arabinose,trehalose,melibiose,sorbitol,aesculin,tartrate,gelatin were identical and 10 items including acetate,D-fructose,valerate,rhamnose,xylose,sucrose,maltose,galactose,starch,mannose were different; 12 items including cytochrome C oxidase,catalase,MR,VP,hydrolysis of aesculin,production of levan,reduction of nitrate,production of urease,phenylalanine,arginine dihydrolase,lecithinase,hydrolysis of gelatin identical and 4 items including hydrolysis of starch,production of indole,production of H_ 2 S,litmus milk different among 16 biochemical ones in strains.The 13 strains could be differentiated by their sensitivities to 3 phages and be divided into 6 types,while no difference could be detected among the strains by the fatty acid profiles.更多还原