【摘要】 目的通过对吗啡依赖大鼠视皮层γ-氨基丁酸(GABA)含量及GAD与GABA-T活力的测定,研究吗啡成瘾过程中视皮层γ-氨基丁酸的代谢变化。方法30只SD大鼠分为6组,末次给药前10 min,后10 min,后3 h处死组及相应对照组,每组5只。纸电泳法检测各组大鼠视皮层中GABA含量,分光光度法检测GAD与GABA-T的活力。结果吗啡末次给药前处死组大鼠GABA含量(0.09±0.03)μmol/mg与对照组(0.17±0.05)μmol/mg比较,差异有统计学意义,末次给药后处死组GABA含量回复至对照组水平。结论吗啡依赖形成时视皮层处于去抑制状态。更多还原

【Abstract】 Objective To investigate the changes of γ-aminobutyric acid(GABA) content and glutamic acid decarboxylase(GAD),γ-aminobutyric acid transaminase(GABA-T) activity in visual cortex of morphine-dependent rats.Methods Paper electrophoresis was employed to detect GABA content in visual cortex and spectrophotometric method was used to investigate the activity of GAD and GABA-T in each experimental group.Results GABA content in visual cortex of rats decapitated before the last morphine injection was significantly decreased(0.09±0.03)μmol/mg compared with saline control(0.17±0.05)μmol/mg.GABA content of rats decapitated after the last morphine injection restored to normal level.Conclusion Visual cortex is in disinhibition with chronic morphine exposure.更多还原