【摘要】 目的:研究胰腺癌肿瘤组织及外周血有核细胞多药耐药基因1(mdr1)及其转录区域结合位点甲基化的表达及其相关性,探索适用于临床观察多药耐药性变化的有效手段。方法:应用免疫组织化学法和FQ-PCR技术、MSP方法分别检测胰腺癌肿瘤组织及其对应外周血标本中的mdr1的表达和转录区域结合位点甲基化程度,并通过Pearson统计学方法分析两者的相关性。结果:胰腺癌P-gp的表达与mdr1mRNA表达明显相关,并与mdr1基因转录区域结合位点甲基化程度显著相关。外周血中mdr1及其转录区域结合位点甲基化的表达与肿瘤组织中的表达存在显著相关性(P<0.01)。结论:mdr1转录区域结合位点的甲基化与胰腺癌的多药耐药性密切相关;检测外周血mdr1及其甲基化的表达可动态监察胰腺癌病人化疗前后多药耐药性的变化,为临床治疗方案的决择提供依据。更多还原

【Abstract】 Objective To investigate the expression of mdr1 mRNA and methylation of DNA transcriptional domain binding site in pancreatic cancer tissues and in peripheral blood.Methods The expression of mdr1 and methylation of DNA transcriptional domain binding site in pancreatic cancer tissues and in peripheral blood were detected by quantitative immunohistochemical analysis,real-time fluorescent quantitative PCR(FQ-PCR),and methylation-specific PCR(MSP),respectively.Pearson method of statistics was used to analyze the correlation.Results The expression of mdr1 was closely related with P-gp expression,and also with methylation of-110GC-box and-50GC-box in mdr1-DNA transcriptional domain binding site.The mdr1 gene expression and methylation in peripheral blood samples were closely related with those of the tumor tissues.Conclusions The methylation of transcriptional domain binding site of mdr1 gene was closely correlated with the status of multidrug resistance in patients with pancreatic cancer.mdr1 gene expression and methylation in peripheral blood might indicate the status of MDR in pancreatic cancer patients prior to and after chemotherapy.更多还原