【摘要】 目的:研究丹参对移植脾再灌注损伤的保护作用及其机制。方法:采用套管法建立大鼠脾移植模型。分别用丹参加乳酸林格液、WMO-1号液、丹参加WMO-1号液灌注保存移植脾:保存2h后重建移植脾血供:再灌注3 h后切取移植脾。比较三组移植脾的阳性凋亡细胞数、细胞间黏附分子-1(ICAM-1,CD54)免疫阳性微血管数、超氧化物歧化酶(SOD)活力值和丙二酰二醛(MDA)的含量。结果:与其他两组相比,丹参加WMO-1号液灌注保存组移植脾的阳性凋亡细胞数、ICAM-1免疫阳性微血管数、MDA含量显著降低(P＜0．01),SOD活力值显著增高(P＜0．01)。结论:丹参通过抗自由基、抑制细胞凋亡、降低ICAM-1表达等机制对移植脾再灌注损伤起保护作用。更多还原

【Abstract】 Objective To evaluate the protective effects of radix salviae miltiorrhizae(RSM)on the ischemia-repeffu- sion injury of spleen graft in the rat,and to explore its possible mechanism.Methods The rat model of spleen transplan- tation was established,and the spleen grafts were perfused and preserved in①RSM+lactated Ringer(LR)solution,②WMO-1 solution,or③RSM+WMO-1 solution respectively for 2 hours;then splenectomy was performed after 3 hours of reperfusion.Immunohistochemical staining and biochemical assays were used to evaluate the status of apoptosis,interce- llular adhesion molecule(ICAM)-1 expression,activity and amount of superoxide dismutase(SOD)and malondialdehyde (MDA).Results Apoptosis,ICAM-1 expression and MDA concentration were significantly decreased in the RSM+WMO-1 solution group than the other groups(P＜0.01).The activity of SOD was significantly increased in the RSM+WMO-1 solution group(P＜0.01).Conclusions RSM+WMO-1 solution demonstrates stronger protective effects on the ischemia-reperfusion injury in the rat model of spleen transplantation.Its possible mechanism might be related to free radical antagonization, apoptosis inhibition and ICAM-1 over-expression.更多还原