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胰腺癌bcl-xL、cyclin D1的表达及其与STAT3的相互关系

Expression of bcl-xL and cyclin D1 in pancreatic carcinoma and their relationship with signal transducer and activator of transcription-3(STAT3)

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【作者】 刘辰裘正军傅德良倪泉兴虞先浚徐近

【Author】 LIU Chen~1 QIU Zheng-jun~2 FU De-liang~1 NI Quan-xing~1 YU Xian-jun~1 XU Jin~1. 1.Department of general surgery,Huashan Hospital,Pancreatic Disease Institute,Fudan University,Shanghai 200040,China; 2.Department of general surgery,The First People’s Hospital,Shanghai Jiaotong University,Shanghai 200080,China

【机构】 复旦大学附属华山医院普外科复旦大学胰腺病研究所上海交通大学附属第一人民医院普外科复旦大学附属华山医院普外科复旦大学胰腺病研究所 上海200040上海200080上海200040

【摘要】 目的:探讨凋亡抑制基因bcl-xL、细胞周期蛋白(cyclin)D1在胰腺癌组织中的表达情况及其与活化的信号传导和转录激活因子-3(phosphorylated signal transducer and activator of transcription-3,PSTAT3)、胰腺癌临床病理特征的相互关系。方法:采用免疫组织化学检测了41例胰腺癌组织和20例正常胰腺组织中PSTAT3、bcl-xL、cvclin D1、Ki67的蛋白表达,用TUNEL法检测41例胰腺癌组织中的凋亡指数(AI)。结果:胰腺癌组织中PSTAT3、bcl-xL和cyclin D1蛋白的表达分别为70.7%(29例)、87.8%(36例)、61.0%(25例),明显高于正常胰腺组织。bcl-xL、cyclin D1蛋白的表达与PSTAT3的表达均呈正相关(P<0.05),PSTAT3的表达与胰腺癌的临床分期、淋巴结转移、肿瘤细胞增殖指数(PI)、AI密切相关(P<0.05)。结论:胰腺癌组织中bcl-xL、cyclin D1的过度表达与PSTAT3密切相关,STAT3信号通路在胰腺癌的增殖凋亡过程中起重要作用。

【Abstract】 Objective To investigate the expression of anti-apoptosis genes bcl-xL and cyclin D1 in human pan- creatic carcinoma tissues,and their relationship with clinico-pathological characteristics including the expression of phos- phorylated signal transducer and activator of transcription-3 (PSTAT3).Methods PSTAT3,bcl-xL and cyclin D1 protein expression in 41 pancreatic cancer tissues and 20 normal pancreatic tissues were detected by immunohistochemical stai- ning.Simultaneously,the proliferative index (PI) and apoptotic index (AI) of the tumor cells were scored by Ki67 and TUNEL staining,respectively.Results The expression rates of PSTAT3,bcl-xL and cyclin D1 in pancreatic carcinoma were 70.7%(29/41),87.8%(36/41) and 61.0%(25/41),respectively,significantly higher than those in the normal pancreatic tis- sues.Positive correlation was found between the expression of bcl-xL,cyclin D1 and PSTAT3 (P<0.05).Furthermore, PSTAT3 expression was closely related with the clinical staging,status of lymph node metastasis,increased PI and decreased AI(P<0.05).Conclusions Activated STAT3 is closely related to the over-expression of anti-apoptosis genes bcl-xL and cy- clin D1 in human pancreatic carcinoma,and might play an important role in the tumorigenesis of this lethal malignancy.

  • 【文献出处】 外科理论与实践 ,Journal of Surgery Concepts & Practice , 编辑部邮箱 ,2007年01期
  • 【分类号】R735.9
  • 【下载频次】157
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