【摘要】 在弱碱性的条件下,叶酸活化酯与牛血清白蛋白（BSA）反应生成叶酸-牛血清白蛋白偶联物（叶酸-BSA）,该偶联物再与二乙三胺五乙酸（DTPA）的酸酐反应,最后与GdCl₃进行螯合制得叶酸-BSA-（Gd-DTPA）n。配合物的结构通过紫外光谱法进行了鉴定,并定量测定了配合物中叶酸、Gd-DTPA对BSA的偶联率。通过测定配合物的体外弛豫时间T₁,进一步分析其弛豫性能R₁。结果表明本研究制得的叶酸-BSA-（Gd-DTPA）_n配合物中叶酸的偶联率约为5,体外弛豫性能R₁约为6×10-3L·mmol^-1·ms^-1,与未偶联叶酸的BSA-（Gd-DTPA）_n的弛豫性能无显著性差异,且比小分子Gd-DTPA的弛豫性能提高了3倍左右。更多还原

【Abstract】 Under weak base condition, Bovine serum albumin (BSA) was reacted with the activated folate firstly; then the conjugates were coupled with Diethylene triamine pentaacetic dianhydride (cDTPAA) to form Folate-BSA-DTPA; finally, these coupled compounds were chelated with GdCl3. All complexes were characterized by UV, the ratios of folate and Gd-DTPA to BSA were determined by UV and ICP-AES methods, respectively. The spin-lattice relaxivity of the Gd complexes in vitro was analyzed. As a result, the ratio of folate to BSA was about 5, and the relaxivity was about 6×10-3 L·mmol-1·ms-1 enhanced three times compared to the small molecular Gd-DTPA, though there was no difference in relaxivity between folate-BSA-(Gd-DTPA)n and BSA-(Gd-DTPA)n.更多还原