【摘要】 采用GC-MS技术,利用2种不同极性的毛细管柱分析了羽裂蟹甲草挥发油的化学组成,确定了31种成分的化学结构与相对含量,结果表明其挥发油主要由单萜、氧化单萜和倍半萜组成,其中倍半萜的含量32.1%尤其显著.α-姜烯(13.49%)、大牦牛儿烯D(10.76%)、α-蒎烯(8.54%)、顺式丁香烯(6.36%)、芳樟醇(6.16%)、β-月桂烯(4.89%)、顺式β-罗勒烯(4.40%)和顺式罗勒烯酮(3.58%)是其主要成分.同时采用滤纸片琼脂平板扩散法和肉汤稀释法测试了其挥发油对2种真菌和12种细菌(包括6种临床致病菌)的抗菌活性.结果表明挥发油对大部分微生物均具有很好的抗菌作用,尤其对酵母菌、革兰氏阳性菌作用更强,其最低抑菌浓度(MIC)为0.16~5.00 g/L,最低杀菌浓度(MBC)为0.16~5.00 g/L.更多还原

【Abstract】 Essential oil of subterranean part of Cacalia tangutica(Maxim.) Hand.-Mazz was analyzed by GC-MS in two different capillary columns of different polarities.The results showed that thirty-one components were identified in the oil,and main compounds of the oil were α-Zingiberene(13.49%),Germacrene D(10.76%),α-Pinene(8.54%),Caryophyllene(Z-)(6.36%),Linalool(6.16%),β-Myrcene(4.89%),β-Ocimene(Z-)(4.40%)and Ocimenone(Z-)(3.58%).The antimicrobial activity of the oil was evaluated against 2 fungi and 12 bacteria including 6 clinical isolated strains using the agar disc diffusion and broth micro dilution methods.Our results showed that the oil presented a broad antimicrobial spectrum,and had better antimicrobial activity against yeast and gram-positive bacteria,the MIC values were 0.16-5.00 g/L and the MBC values was 0.16-5.00 g/L.更多还原