【摘要】 目的:从肾细胞凋亡角度揭示耐力训练提高机体抗疲劳能力的机制。方法:24只雄性SD大鼠随机分为控制对照组(C)、力竭运动组(LE)、耐力训练力竭运动组(ET),于力竭运动后24 h取材,肾脏凋亡细胞检测采用TUNEL法,Bcl-2、Bax蛋白表达检测采用免疫组化法,并检测NO生成能力。结果:ET组力竭运动时间显著延长(P<0.01),Bcl-2蛋白表达明显高于C组和LE组(P<0.01),NOS活性显著低于C组(P<0.05)、非常显著低于LE组(P>0.01);各组的肾细胞凋亡指数、Bax蛋白表达、Bax/Bcl-2和NO浓度差异不显著(P<0.05)。结论:耐力训练机体抗疲劳能力提高,与恢复期肾组织细胞Bcl-2蛋白表达增高、NOS活性降低、NO浓度水平正常,凋亡细胞少有关。更多还原

【Abstract】 The purpose of this study was to observe possible mechanism that endurance training can enhance anti-fatigue capability by investigating renal cell apoptosis.Twenty-four male Spragne-Dawley rats were randomly divided into control group(C),low intensity exhaustive exercise group(LE)and endurance training group(ET).The kidney tissue was sampled at 24 hour after exhaustive exercise.The apoptosis,Bcl-2 and Bax protein expression of renal cell was investigated with TUNEL and immunohistochemistry method.The result showed that In ET group,rat exhaustive exercise time was significantly prolonged(P<0.01).Compared with C and LE groups,Bcl-2 protein expression of renal cell was obviously increased(P<0.01),the NOS activity was decreased(P<0.05～0.01).The rest were insignificantly different between three groups(P<0.05).It concludes that endurance-training can enhance anti-fatigue capability through increasing Bcl-2 protein expression,decreasing NOS activity,obtaining NO content and inhibited apoptosis caused by exhaustive exercise in kidney tissue.更多还原