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乐斯本降解真菌的筛选及降解特性研究

Isolation and degradation characteristics of chlorpyrifos-degrading Fungi

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【作者】 秦坤唐心强张丽青

【Author】 QIN Kun,TANG xin-qiang,ZHANG li-qing (Taishan Medical College,Taian 271000,Shandong,China)

【机构】 泰山医学院泰山医学院 山东泰安271000山东泰安271000

【摘要】 目的从污水排放口的污泥中分离获得能够降解乐斯本的真菌,并研究其降解特性。方法用紫外分光光度法和总量法测定了外加碳源浓度、初始pH值、乐斯本浓度对真菌的降解能力和生长量的影响。结果分离得到三株降解乐斯本的真菌,三株真菌5 d内对50 mg.L-1乐斯本的降解率分别高达93.53%、91.37%、83.45%。结论三株真菌均能以乐斯本为唯一碳源生长从而将其降解,且当乐斯本浓度为20~100 mg.L-1,pH值为6.5~9.0时,真菌的降解效果较好;当乐斯本浓度为50 mg.L-1,pH值为6.5~8.0时,真菌的生长量达到最大。

【Abstract】 Objective: To obtain the chlorpyrifos-degrading fungi isolated from sludge at sewage outlet by enrichment and study the degradation characteristics of the fungi.Methods: The effects of carbon source,pH,chlorpyrifos concentration on the ability of degradation and the fungi growth amount were determined by ultraviolet spectrophotometry and gravimetric method.Results: Three chlorpyrifos-degrading fungi were isolated,the degradation rates of chlorpyrifos with concentration of 50mg·L-1 by these fungi were 93.53%,91.37% and 83.45% in 5 days respectively.Conclusion: These three fungi,with chlorpyrifos as sole carbon source,can degrade chlorpyrifos.The biodegradation rates arre higher when chlorpyrifos concentration is 20~100mg·L-1,pH is 6.5~9.0.The growth amount of the fungi is the highest when chlorpyrifos concentration is 50mg·L-1,pH is 6.5~8.0.

【关键词】 乐斯本降解真菌生长量
【Key words】 chlorpyrifosdegradationfungigrowth amount
  • 【文献出处】 泰山医学院学报 ,Journal of Taishan Medical College , 编辑部邮箱 ,2007年06期
  • 【分类号】X172
  • 【被引频次】10
  • 【下载频次】48
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