【摘要】 目的利用诱导痰技术对一些肺功能正常的吸烟者进行痰内细胞因子检测,以期了解吸烟和慢性阻塞性肺病的关系。方法选取33例符合标准的研究对象,随机分为2组,所有研究对象均于晨7~9时刷牙、漱口;测定基础肺通气功能;4%高渗盐水雾化吸入20分钟,15分钟后复测肺通气功能,每次咳痰前彻底清理口腔、鼻腔分泌物,痰液收集于无菌容器中;收集的痰液标本在1小时内处理,处理前保存于4°C冰箱。用酶联免疫吸附法测定诱导痰上清液中IL-8、IL-10、IFN-γ及TNF-α。结果IL-8水平明显上升,而IL-10水平显著降低。结论吸烟者在临床上尚无呼吸道症状时便已经出现痰内细胞因子的变化,亦提示慢性气道炎症业已存在。这可能是吸烟作为慢性阻塞性肺病(COPD)的主要诱发因素之一,在COPD气道炎症的形成中起重要作用的原因。更多还原

【Abstract】 Objective: To explore the relationship between the smoking and chronic obstractive pulmonary disease(COPD) by testing cell factor in phlegm among some smokers with normal pulmonary function,using the phlegm-induced technique.Methods: 33 sbjects were selected according to standards,and divided into 2 groups at random.All of the subjects were to brush teeth and gargle at 7:00-9:00 in the morning;the function of foundational lung ventilation was measured;4% fog of hypsotonic brine was inhaled in 20 minutes,then the function of lung ventilation was remeasured after 15 minutes;the mouth and nose secretion were cleared thoroughly each time before spiting out phlegm,then the phlegm was collected in nongerm container;the phlegm liquid specimens was processed within an hour,before witch,the specimens were kept in 4 °C refrigerator.IL-8,IL-10,IFN-γ and TNF-α in the superior clear liquid of phlegm-induced were tested using Enzyme Linked Immuno Sorbent Assay.Results: The level of IL-8s increased obviously,but the level of IL-10s droped obviously.Conclusion: It suggests that the smokers without respiratory symptom clinically have already had the changes of cell factors in phlegm,and there also exists the chronic airway inflammatory and that may be why smoking is acted as one of the main inducement of COPDand plays an immportant role in the formation of COPD.更多还原