【摘要】 以曼氏袋鼠爪(Anigozanthos manglesii)的根颈、花莛、叶为外植体,接种于不同浓度激素配比的MS培养基上。结果表明:根颈是曼氏袋鼠爪组培快繁的最适外植体;诱导芽生长的最适培养基为MS+6-BA 2.0 mg/L+NAA 0.1 mg/L,诱导率为93.3%;芽增殖的最适培养基为MS+6-BA 0.5 mg/L+NAA 0.1 mg/L,增殖倍数为7;诱导生根的最适培养基为1/2 MS+6-BA 0.01 mg/L+NAA 0.1 mg/L,每苗生根数为6～8个;蛭石+草炭土+河沙(2∶2∶1)是组培苗成活及生长的最适基质配比,成活率达98%。更多还原

【Abstract】 The root neck,scape and foliage of Anigozanthos manglesii were used as explants,which were inoculated to the MS substrate with different hormonal concentration.The results show that the root neck is the best explant of tissue culture and fast propagation for Anigozanthos manglesii.The best substrate to induce the growth of sprout is MS+6-BA 2.0 mg/L+NAA 0.1 mg/L,and the inductivity is 93.3%.The most suitable substrate for proliferation of bud is MS+6-BA 0.5 mg/L+NAA 0.1 mg/L,and it proliferated 7 times.The best substrate to induce root is 1/2 MS + 6-BA 0.01 mg/L + NAA 0.1 mg/L,and every seedling proliferates 6～8 roots.The best substrate for living and growing of tissue culture seedling is compound of vermiculite,plant ash and sand(2∶2∶1),and the survival rate reaches 98%.更多还原