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半边旗药材超声提取工艺的研究

Studies on Ultrasonic Extraction Process in Pteris semipinnata L.

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【作者】 典灵辉梁念慈龚先玲

【Author】 DIAN Ling-hui,LIANG Nian-ci, GONG Xian-ling(Guangdong Medical College,Key Laboratory of Natural Drug Research and Development of Guangdong,Zhanjiang 524023,China)

【机构】 广东医学院广东省天然药物研究与开发重点实验广东医学院广东省天然药物研究与开发重点实验 广东湛江524023广东湛江524023

【摘要】 目的优选半边旗的超声提取工艺。方法采用L9(34)正交实验,考察乙醇浓度(A)、提取时间(B)、提取次数(C)和料液比(D)4个影响因素,以5 F含量为评价指标,并采用薄层扫描法进行测定。结果半边旗超声提取的最佳工艺为A2B2C3D3,即加10倍量70%乙醇,超声提取3次,30 min/次。结论优选得到的工艺稳定可行。

【Abstract】 Objective To optimize the conditions of ultrasonic extraction of Pteris semipinnata L.Methods The four factors-alcohol content(A),ultrasonic time(B),ultrasonic times(C) and solvent volume(D) were investigated by L9(34) orthogonal design,and the content of 5F were defined as the standard index.5F was determined by TLC-Scanning.Results A2B2C3D3 was the optimum condition of the ultrasonic extraction,which was adding 10 times amount of 70% alcohol and extracting for 30 min(totally extracting for 3 times).Conclusion The optimized process is stable and available.

【基金】 广东省湛江市科技计划攻关项目;广东省科委大科技攻关项目(No.9622007002)
  • 【文献出处】 时珍国医国药 ,Lishizhen Medicine and Materia Medica Research , 编辑部邮箱 ,2007年06期
  • 【分类号】R284.2
  • 【被引频次】1
  • 【下载频次】108
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