【摘要】 目的建立HPLC法测定以水煎法提取19种中成药中甘草酸(GA)的含量。方法采用日本岛津PC8色谱柱;流动相为甲醇-水-醋酸(57.0∶42.6∶0.4);检测波长为254 nm;流速2.0 ml.min-1。结果甘草酸在200-2000μg.ml-1,r=0.999 7的浓度范围内线性关系良好,平均回收率为96.55%,RSD=1.1%;同时研究了22种单味药对甘草中GA含量测定的影响。结论通过与冷浸法比较,水煎法和冷浸法提取中成药中GA含量差值较大,且水煎法的偏低。更多还原

【Abstract】 Objective An HPLC method was established for the determination of glycyrrhizic acid(GA) by extracting method of decoction in 19 kinds Chinese patent medicine prepared in laboratory.Methods PC8 column was used with methanol-watercuetu acid(57.0∶42.6∶0.4) as mobile phase.The flow rate was 2.0ml·min-1 and detection wavelength was 254nm.Results The linear relationship was good when the content of glycyrrhizic acid(GA) was in the range of 200～2 000 μg·ml-1(r=0.999 7).The average recovery and RSD were 96.55% and 1.1% respectively.The paper also studied the interference determination of GA by selecting interrelation 22 kinds of single medicinal material.Conclusion By comparison the decoction with the infusion,the results shows that the content of GA by extraction decoction was lower than that by infusion.更多还原