【摘要】 目的探讨小柴胡汤对内异症大鼠异位内膜细胞凋亡的影响。方法建立大鼠内异症模型,采用透射电镜技术及免疫组化方法,观察小柴胡汤对异位内膜细胞形态结构和Fas及Caspase-3蛋白表达的影响。结果中、高剂量小柴胡汤(10,15 g.kg-1.d-1)治疗后,异位内膜有较多腺上皮细胞出现凋亡的特征,表现为细胞体积变小,核固缩,胞浆和核染色质凝集,密度增高,细胞间凋亡小体,同时间质细胞中可见一些坏死细胞;异位内膜Fas蛋白、Caspase-3蛋白的表达水平明显高于其在位内膜。结论小柴胡汤可通过上调Fas蛋白的表达,诱导异位内膜细胞的凋亡。更多还原

【Abstract】 Objective To explore the effect of XiaoChaiHu Decoction on ectopic endometrium cell apoptosis in rats.Methods Using the rat model,ectopic endometrium was evaluated by electron microscope.Fas and Caspase-3 protein expression in the endometrium and endometriotic tissue were observed by immunohistochemical method.Results The glandular cell in the endometrial implant after therapy with middle or high-dose of(10,15 g·kg-1·d-1) XiaoChaiHu Decoction showed characteristic features of apoptosis which displayed by the cell decreased in size,karyopyknosis,cytoplasmic and nuclear chromatin condensation,density increased and a lot of apoptotic bodies among cell.Whereas some stromal cell displayed degeneration and necrosis.The protein expression of Fas and Caspase-3 in endometriotic tissue of XiaoChaiHu Decoction group was higher than that in the endometrium.Conclusion The therapeutic mechanism of XiaoChaiHu Decoction for endometriosis may be related to promoting the apoptosis in endometriotic tissue by increasing Fas protein expression.更多还原