【摘要】 目的:克隆和鉴定子痫前期胎盘组织差异表达基因,确定与子痫前期相关的基因。方法:应用荧光mRNA差异显示技术寻找并克隆子痫前期胎盘组织差异表达的基因,对其中的ATP结合盒转运子G₂（ATP binding cassette transporter G₂,ABCG₂）基因用半定量RT-PCR技术验证其表达。结果:ABCG₂基因在子痫前期胎盘组织中高表达,半定量RT-PCR结果与mRNA差异显示的结果相符合,ABCG₂基因在子痫前期胎盘组织中的表达高于正常胎盘组织,差异有显著性（P<0.05）。结论:荧光mRNA差异显示技术可用于筛选子痫前期胎盘组织差异表达基因,并应用此技术发现了ABCG₂基因在子痫前期患者胎盘组织表达水平增高。更多还原

【Abstract】 Objective: To clone and identify the genes differentially expressed in placentas of preeclampsia and determine which involved in pathogenesis of preeclampsia. Methods: Fluorescent differential display (FDD) was used to find and clone the differentially expressed genes in placentas of preeclampsia, the expression of the ATP binding cassette transporter G2 (ABCG2 ) gene was verified by semi-quantitive reverse transcription- polymerase chain reaction(RT-PCR). Results: The ABCG2 gene was found by FDD and its expression was up-regulated in placentas of preeclampsia, it was confirmed by RT-PCR that the mRNA expression of ABCG2 gene was significantly higher in placentas of preeclampsia than that in the matched ones(P<0.05). Conclusion: FDD can be used for screening differentially expressed genes in placentas of preeclampsia.更多还原