【摘要】 目的:了解白藜芦醇诱导大鼠鼻咽癌细胞CNE-2 Z凋亡过程中半胱天冬酶-6(caspase-6)的活化情况。方法:采用实时PCR检测caspase-6 mRNA表达的改变,比色法测定caspase-6活性的变化。结果:采用不同浓度的白藜芦醇(0μmol/L、30μmol/L、60μmol/L、120μmol/L、240μmol/L)处理小鼠鼻咽癌细胞24h,caspase-6 mRNA的表达呈浓度依赖性的增加(P<0.01);caspase-6活性呈浓度和时间依赖性的升高(P<0.01)。结论:白藜芦醇诱导小鼠鼻咽癌细胞CNE-2Z凋亡过程中存在caspaso-6的活化。更多还原

【Abstract】 Objective To investigate whether caspase-6 was activated during resveratrol-induced apoptosis of rats’nasopharyngeal carcinoma cells CNE-2Z.Methods The changes of caspase-6 mRNA was detected by semi-quantitative RT-PCR.The changes of caspase-6 activity was determined by colorimetric assay.Results After CNE-2Z cells were treated with 0 μmol/L,30 μmol/L,60 μmol/L,120 μmol/L,240 μmol/L resveratrol for 24 hours,respectively,the expression of caspsae-6 mRNA was increased in a concentration-dependent manner(P<0.01).The caspase-6 activity was increased in a concentration and time-dependent manner(P<0.01).Conclusion Caspase-6 is activated during resveratrol-induced apoptosis of CNE-2Z cells.更多还原