【摘要】 目的分析吉林地区汉族人群两个短串联重复序列(STR)位点即D5S818和D19S400的遗传多态性分布,获得群体遗传学数据。方法从吉林地区103名汉族无血缘关系的个体抗凝血样中提取DNA,进行PCR扩增,非变性聚丙烯酰胺凝胶电泳分型并银染。结果得到两个基因座各等位基因的基因频率,基因型频率分布符合Hardy-Weinberg平衡。杂合度和多态性信息含量较大。结论这两个基因座具有高度多态性,是较理想的遗传标记。所得到的遗传学数据可为法医学个人识别、亲子鉴定及群体遗传学研究提供依据。更多还原

【Abstract】 Objective To analyze the genetic polymorphism of two short tandem repeat(STR)loci,namely,D5S818 and D19S400 and to obtain the corresponding genetic data.Methods DNA was abstracted from the blood cells of 103 unrelated individuals of han population.Two loci were amplified with PCR method.The PCR products were analyzed by PAGE,followed by silver staining.Results The allele frequency of two loci was obtained.The genotypes distributions of the loci were consistent with Hardy-Weinberg equilibrium and had higher heterozygosities and PIC.Conclusion The two STR loci have higher polymorphism and are better genetic markers.The genetic data of these two STR loci may be used in individual identification,paternity testing and in other population genetic researches.更多还原