【摘要】 目的探讨肿瘤坏死因子相关凋亡诱导配体(TRAIL)对人C-erbB2过表达的乳腺癌SKBR-3细胞株的作用及与赫赛汀(Herceptin)联用的可行性。方法SKBR-3细胞培养贴壁后,加入不同浓度的TRAIL、Herceptin、TRAIL联合Herceptin,MTT法检测抑制率,流式细胞术检测凋亡率。结果乳腺癌SKBR-3细胞对TRAIL不敏感,1μg/ml的TRAIL只能杀伤(5.54±1.12)%的细胞,TRAIL与Herceptin联用可增加对肿瘤细胞的抑制率,10、25μg/mlHer-ceptin与1μg/mlTRAIL联用的抑制率分别为(27.82±8.90)%、(36.89±7.60)%,与两药单用相比具有明显差异(均P<0.05)。10μg/mlHerceptin与1μg/mlTRAIL联用的凋亡率为(21.55±3.96)%,与两药单用相比具有明显差异(均P<0.05)。结论乳腺癌SKBR-3细胞株对TRAIL不敏感,但TRAIL与Herceptin联用可增加抗肿瘤作用,可能成为一种对C-erbB2过表达乳腺癌的新的治疗方法。更多还原

【Abstract】 Objective To investigate the anti-tumor effects of TRAIL and TRAIL combined with Herceptin in breast cancer cell lines SKBR-3.Methods TRAIL,Herceptin and TRAIL combined with Herceptin were added at different concentration at different scheduled SKBR-3 cells incubation time,MTT working solution was added and the inhibition was calculated.The rate of apoptosis was detected by flow cytometry.Results SKBR-3 cells were not sensitive to TRAIL,the rate of killing was(5.54±1.12)%with TRAIL at concentration of 1 μg /ml.TRAIL combined with Herceptin upregulated the inhibition.The inhibition was(27.82±8.90)%,(36.89±7.60)%when 10,25 μg/ml Herceptin combined with 1 μg /ml TRAIL was added,for which there was significant difference with that in the group using TRAIL or Herceptin alone.The rate of apoptosis in the group with 10 μg /ml Herceptin combined with 1 μg /ml TRAIL was(21.55±3.96)%,which also showed significant difference compared with that in the group using TRAIL with Herceptin alone.Conclusion TRAIL combined with Herceptin can effectively inhibit breast cell lines SKBR-3 even these cells are resistant to TRAIL alone,which may be a new therapeutic method for treatment of C-erbB2 overexpression breast cancer.更多还原