【摘要】 目的探讨还原型烟酰胺腺嘌呤二核苷酸(NADH)脱氢酶亚单位1、2、5在早产大鼠高氧肺损伤组织中的动态表达及其意义。方法早产新生大鼠生后1d随机分为高氧和空气组,高氧组持续暴露于850mL/L氧气中,空气组置同一室内常压空气中。分别取二组大鼠高氧或空气暴露后1、4、7、10和14d肺组织标本,采用HE染色观察肺组织形态学改变,采用RT-PCR法在mRNA水平检测NADH脱氢酶亚单位1、2、5的表达。结果1.高氧暴露1、4d,早产大鼠肺组织形态较空气组无明显改变;高氧暴露7、10d,肺组织渐出现小血管充血扩张,肺泡腔内炎性细胞浸润,肺泡间隔增厚,肺泡数目减少、结构简单化和囊泡化等改变。2.空气组NADH脱氢酶亚单位1(ND1)和亚单位2(ND2)mRNA的表达随日龄增长渐降低,至7d时最低,随后其表达逐渐增高;空气组NADH脱氢酶亚单位5(ND5)mRNA的表达随日龄增长渐降低,至4d时最低,随后其表达逐渐增高。3.与同时间点空气组相比,高氧暴露后1、4和7dND1和ND5mRNA表达显著增强(Pa<0.05),随后其表达渐下降,10、14d时低于空气组,但二者相比差异无显著性意义(Pa>0.05);与同时间点空气组相比,高氧暴露后1、4d ND2 mRNA表达二组间差异无显著性意义(Pa>0.05),7d时较空气组极显著增强(P<0.01),10、14d时较空气组显著降低(Pa<0.05)。结论高氧可导致早产大鼠肺组织NADH脱氢酶表达改变,提示其可能参与高氧肺损伤的病理生理过程。更多还原

【Abstract】 Objective To explore the temporal expression of reduced nicotinamide adenine dinucleotide(NADH) dehydrogenase subunit 1,2,5 in lung of premature rats with hyperoxia-induced lung injury and its significance.Methods Premature Sprague-Draw rats were randomly assigned to hyperoxia group and air group at 1 d after birth.These rats in hyperoxia group were persistently exposed to 850 mL/L oxygen and those in air group to room air.The lung tissues of both groups were collected at 1,4,7,10 and 14 d after being exposed to hyperoxia or air.The lung histological changes were observed by HE staining,and the expression of NADH dehydrogenase subunit 1,2,5 mRNA were detected by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR).Results 1.There were no lung histological changes in hyperoxia group before the 4 d after being exposed to hyperoxia,then the lung in hyperoxia group gradually existed a series pathological histological changes.2.The expressions of NADH dehydrogenase subunit 1(ND1) and subunit 2(ND2) mRNA in air group gradually decreased with age,the lowest at 7 d,then it gradually increased;the expression of NADH dehydrogenase subunit 5(ND5) mRNA in air group gradually decreased with age,the lowest at 4 d,then it gradually increased.3.Compared with air group,the ND1 and ND5 mRNA levels in hyperoxia group were significantly higher (Pa<0.05) at 1,4 and 7 d,then it gradually decreased,lower than air group at 10 and 14 d,but there was no significant difference(Pa>0.05);compared with air group,the ND2 mRNA level in hyperoxia group had no obvious changes at 1 and 4 d,increased significantly at 7 d (P<0.05),and then decreased significantly at 10 and 14 d (Pa<0.05).Conclusion Hyperoxia induces the expression of NADH dehydrogenase in premature rat lung,this indicates that this may participate in the physiological and pathology processes of hyperoxia-induced lung injury.更多还原