【摘要】 目的探讨癫(EP)患儿血清和脑脊液(CSF)神经元特异性烯醇化酶(NSE)和肿瘤坏死因子-α(TNF-α)的表达及其与脑损伤的关系。方法EP患儿62例,分为严重组28例(首次采集标本前24 h内发作≥3次和(或)单次发作时间≥15 min),轻症组34例(24 h内发作<3次及单次发作时间<15 min)。采用电化学发光法测定62例EP患儿惊厥发作后24 h内和30例对照组血清和CSF中NSE水平;采用放射免疫法测定其血清NSE和CSF中TNF-α水平。结果EP严重组、轻症组和对照组血清和CSF中NSE、TNF-α经方差分析均有显著性差异(Pa<0.01)。严重组血清和CSF中NSE、TNF-α明显高于轻症组(P<0.01,0.05)。二组血清和CSF中NSE、TNF-α与对照组比较,均有显著性差异(Pa<0.01)。NSE血清和CSF水平有相关性[r(重)=0.579,r(轻)=0.55Pa<0.01];TNF-α水平也有相关性[r(重)=0.525,r(轻)=0.488 Pa<0.01]。CSF中NSE、TNF-α水平呈高度正相关[r(重)=0.512,r(轻)=0.454 Pa<0.01]。结论EP患儿血清、CSF中NSE和TNF-α水平明显增高,NSE上升水平与癫发作的频率、持续时间密切有关,能反映癫发作脑损伤的严重程度。TNF-α与NSE相关,参与EP脑损伤的病理过程。更多还原

【Abstract】 Objective To explore the levels of neuron-specific enolase(NSE) and tumor necrosis factor-α(TNF-α)concentrations of serum and cerebrospinal fluid(CSF) in children with epilepsy,and evaluate its relationships with neuronal damage.Methods Sixty-two epilepsy children were divided into 2 groups:severe group including 28 cases of frenquent seizures ≥3 vices or time of master single test seizures≥15 min,mild group including 34 cases of infrenquent seizures <3 vices or time of master single test seizures time<15 min.The serum and CSF NSE(r=0.579,severe group;r=0.550,mild group Pa<0.05) and TNF-α(r=0.525,severe group;r=0.488,mild group Pa<0.01) were determined respectively by electrochemiluminescenc and radioimmunoassay in 62 epilepsy children within 24 h after seizures and 30 controls.Results The serum and CSF levels of NSE and TNF-α had significant difference among severe group,mild group and control group through variance analysis(Pa<0.01).The serum and CSF levels of NSE and TNF-α in severe group within 24 hours after seizure were signi-ficantly higher than those in mild group(P<0.01,0.05).The levels of NSE and TNF-α in severe group or mild group were significantly higher than those in control group(Pa<0.01).The serum NSE(r=0.579,severe group;r=0.55,mild group Pa<0.01) and TNF-α(r=0.525,severe group;r=0.488,mild group Pa<0.01) were positively correlated with those of CSF,respectively.The CSF levels of NSE were positively correlated with CSF levels of TNF-α(r= 0.512,severe group;r =0.454,mild group Pa<0.01).Conclusions The serum and CSF NSE and TNF-α significantly increase in epilepsy children.Ascendant level of NSE is correlated to frequency and duration of seizure,and can reflect severe degree of brain injury after seizure.TNF-α is positively correlated with NSE,and participate in pathoprocedure of brain injury after seizure.更多还原