【摘要】 目的观察铁剥夺对白血病细胞增殖的影响。方法以K562细胞作为人类白血病细胞株,台盼蓝染色,光镜下计数活细胞率;用四甲基偶氮唑蓝(MTT)法测A值(570nm),绘制生长曲线;流式细胞术分析细胞周期变化。结果小剂量去铁胺(DFO)(12.5μmol/L)处理K562细胞时,生长曲线轻微变化,随时间延长和DFO剂量增加(25、50、100μmol/L),细胞存活率明显下降,生长曲线高峰显著低于对照组。DFO的抗增殖活性为时间-剂量依赖型。用DFO(50、100μmol/L)处理K562细胞48和72h后,G0/G1期细胞数量增加,S期细胞数量减少,与对照组比较均有显著性差异(Pa<0.001)。上述作用可被等浓度的三氯化铁抵消。结论铁剥夺具有抗白血病细胞增殖作用,剥夺细胞内铁,阻止细胞由G0/G1期进入S期可能是其机制之一。更多还原

【Abstract】 Objective To observe the antiproliferative activity of iron-deletion on K562 cell.Methods K562 cell was used as human leukemia cell lines and treated with desferrioxamine in different dosages,the antiproliferative effect of iron-deprivation was observed by methyl thiazolyl tetrazolium(MTT),and the cell cycle analysis was performed by flow cytometry.Results When K562 cells were treated with low-dose desferrioxamine(DFO)(12.5 μmol/L),the growth curve changed slightly,but the peaks of the growth curve were significantly lower than those of control group when K562 cells were treated with mediate-dose(25 μmol/L) and high-dose DFO(50 μmol/L).The antiproliferative activity of DFO increased in a dose dependent manner.The proportion of cells in G0/G1 phase increased and that in S phase decreased,which was significant different from that of control group(Pa<0.001) when K562 cells were treated with DFO(25 μmol/L,50 μmol/L) after 24 and 72 h.Conclusions Iron-deprivation may have antiproliferative activity of leukemic cell.One of the mechanisms may be carried out by chelating intracellular iron and blocking the entrance of cells from G0/G1 to S phase.更多还原