【摘要】 通过正交试验确定了L-乳酸高产菌株HT的最适发酵培养基为:麦根4%、磷酸氢二钾0.5%、硫酸铵0.5%、豆饼粉0.5%、玉米淀粉水解糖5%、碳酸钙5%、pH值为6.5;通过变温培养试验确定其最适的生长温度为28℃,最适的发酵温度为46℃;采用优化后的发酵培养基进行L-乳酸发酵,HT菌株在第4天就到达了发酵终点,L-乳酸产量为40.19g.L-1,对糖的转化率为92.6%。更多还原

【Abstract】 The optimum fermentation conditions of the strain HT were studied.Its optimum growth temperature was 28℃,fermentation temperature was 46℃,the optimum pH was 6.5.According to the experimental results of fermentation conditions,the optimum medium were as follows: barley root 4%,K2HPO4 0.5%,(NH4)2SO4 0.5%,bean powder 0.5%,starch hydrolytic sugar 5%,CaCO3 5%?Under these fermentation conditions,the days of fermentation was four,the yield of L-lactic acid was 40.19g.L-1,and the conversion ratio of substrate was 92.6%.更多还原