【摘要】 目的探讨N5,N10-亚甲基四氢叶酸还原酶(MTHFR)基因多态性与冠心病的关系。方法对2003年11月至2005年8月贵阳医学院附属医院及贵州市第二人民医院住院的87例冠心病患者(冠心病组)及同期在门诊进行健康体检的73名健康人(对照组),采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)法检测其MTHFRC677T基因多态性。结果对照组与冠心病组MTHFR677位T等位基因的分布频率分别是18.5%,36.1%,病例组MTHFR基因C677T的CT基因型及T等位基因频率显著高于对照组,差异有显著性(P<0.05)。结论MTHFRC677T基因多态性与冠心病密切相关。更多还原

【Abstract】 Objective Study on methylenetetrahydrofolate reductase(MTHFR)gene polymorphism in coronary heart disease.Methods Detect the MTHFR C677T gene polymorphism by PCR-RFLP in 73 cases of healthy individuals and 87 cases of coronary heart diseas(CHD).Results At the site of 677,the T allele frequencies are 18.5%,36.1%,respectively,for healthy individuals and 87 cases of coronary heart disease.The frequecies of MTHFR CT genotype and T allel were significantly higher in disease groups.There are significant differences between disease group and control group(P<0.05).Conclusion MTHFR C677T gene polymorphism is associated with coronary heart disease.更多还原