【摘要】 目的:探讨祛瘀散结胶囊中人参皂苷Rg1和人参皂苷Rb1的含量测定方法。方法:采用高效液相色谱法测定人参皂苷Rg1和人参皂苷Rb1的含量,色谱柱Hanbon Science & Technology Co.,Ltd Lichrospher NH2柱,乙腈-水(77∶23)为流动相,检测波长203nm,流速1.0mL·min-1。结果:该方法线性关系良好,人参皂苷Rg1和人参皂苷Rb1的线性范围分别为0.0503～0.5026mg.mL-1和0.0504～0.5040mg·mL-1,相关系数分别为0.9999和0.9996,平均回收率分别为100.63%和98.13%,RSD%分别为0.87%和1.25%(n=6)。结论:该方法精密度高、分离度良好,稳定性、重现性好,可用于祛瘀散结胶囊的质量控制。更多还原

【Abstract】 HPLC is adopted to establish a method to determine the content of Ginsenoside Rg1 and Ginsenoside Rb1 in stasis-dissolving capsule. The system consists of NH2 column while acetonitrile -water(77∶23)is a mobile phase with a flow rate of 1.0 ml min and the UV detection wavelength is 203 nm. A good linear correlation has been found in HPLC. As for ginsenoside Rg1, the average recovery is 100.63 %, RSD is 0.87 % (n=6) , and the linear range is 0.050 3～0.502 6 mg·mL (r= 0.999 9); as for ginsenoside Rg2, the average recovery is 98.13 %, RSD is 1.25 % (n=6) , and the linear range is 0.050 4～0.504 0 mg·mL(r=0.999 6).The paper comes to the conclusion that this method is accurate and can be used for quality control of the stasis-dissolving capsule.更多还原