【摘要】 目的:研究rhVEGF对成骨细胞增殖和分化能力的影响。方法:采用细胞培养技术,应用光镜、MTT法及PNPP偶氮法,分别从细胞形态、细胞增殖及分化等特性,分析不同浓度的rhVEGF对成骨细胞增殖和碱性磷酸酶活性的影响。结果:rhVEGF能促进成骨细胞的增殖和分化,其中,在加入3.125ng/ml浓度的rhVEGF并于第3天时,成骨细胞增殖最显著;而12.5ng/ml组对碱性磷酸酶活性的影响最明显。结论:一定剂量的rhVEGF可明显促进成骨细胞的增殖和分化。更多还原

【Abstract】 Objective:To study the effects of recombinant human vascular endothelial growth factor on proliferation and differentiation of rat osteoblasts. Methods: Cell culturing, light microscop, MTT assay and PNPP assay were performed to observe the effects of rhVEGF on proliferation and differentiation of the cells. Results: rhVEGF could stimulate osteoblasts proliferation and differentiation. The maximal effect on proliferation was observed at a concentration of 3.125 ng/ml on the third day. ALP activities in osteoblasts were increased most significantly at a concentration of 12.5 ng/ml. Conclusion: rhVEGF may not only induce angiogenesis to facilitate bone formation, but also stimulate this process in a direct way by inducing proliferation and differentiation of osteoblasts.更多还原