【摘要】 为了筛选在临床上可以有效防治动物氟中毒病的复方中草药制剂,本研究分别对前期研究的复方当归散基础组方加以改进,拟定出5种组方,并进行了抗氟作用研究。试验期间跟踪观察氟斑牙发生情况,测定骨氟含量以及血浆、肝、肾的总抗氧化力(T-AOC)、总抗氧化物歧化酶(T-SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)活力和丙二醛(MDA)含量,建立阳性组氟中毒模型,筛选较为理想的抗氟复方当归散。结果表明:阳性对照组成功复制出氟中毒模型;各给药组药效比较结果表明,抗氟复方当归散Ⅱ较其它组方更能显著降低骨氟含量,较好地抑制大鼠体内的脂质过氧化过程,提高大鼠抗氧化酶活性。更多还原

【Abstract】 ObjectiveTo carry out comparative studies on pharmacological effects five kinds of antifluorine Chinese herbal compound powder formulated according to the theories of the modification based on flavor and action of different Chinese herbs,and establishing of therapeutic principles based on syndrome differentiation in traditional Chinese medicine.Methods All the Wistar rats were randomly divided into 7 groups,namely,5 therapeutic groups with anti-fluorine Chinese herbal compound powder,negative control group,and positive group.The rats of the negative group drank freely normal tap water and the rats of the other groups drank the water containing 100 mg fluorine per liter.Five kinds of anti-fluorine Chinese herbal compound powder were orally administered to the rats of therapeutic groups at a dose of 0.1g/kg respectively.The dental fluorosis was observed and content of fluorine in bone,malondialdehyde levels and activities of total superoxide dismutase,catalase,and glutathione peroxidase in plasma,liver,and kidney were determined when finishing of the test.Results The fluorosis was found only in the rats of positive group.The anti-fluorine Chinese herbal compound powder could obviously decrease the content of fluorine in bone,inhibit significantly the lipid peroxidation and enhance the antioxidase activity in the rats.Conclusion The anti-fluorine Chinese herbal compound powder could depress the generating of fluorosis by inhibiting the lipid peroxidation and enhancing the antioxidase activity in the rats.更多还原