【摘要】 目的:探讨内皮抑素对人宫颈癌Hela细胞生长的抑制作用及其作用机制。方法:用MTT法检测细胞生长;用透射电镜观察细胞凋亡;用免疫细胞化学、RT-PCR及Western blot法检测细胞中bcl-2和bax蛋白及mRNA的表达。结果:内皮抑素抑制Hela细胞增殖;能诱导Hela细胞凋亡;而对细胞中bcl-2和bax蛋白及mRNA的表达无明显影响。结论:人内皮抑素具有抑制宫颈癌Hela细胞生长的作用,其作用机制可能与诱发细胞凋亡相关。更多还原

【Abstract】 Objective:To evaluate the inhibitory effect of endostatin on uterine cervix cancer cell line Hela and to investigate the possible mechanism of the inhibition.Methods:The effect of endostatin on Hela cell proliferation was studied by means of MTT.The cell apoptosis was detected by transmission electron microscopy.Then bcl-2 and bax expressions were measured in Hela cells treated by endostatin by immunocytochemistry,RT-PCR and Western blot method.Results:Endostatin inhibited Hela cell proliferation(P<0.01) and induced cell apoptosis,whereas bcl-2 and bax expression were not changed obviously in Hela cell treated by endostatin.Conclusion:Endostatin might have its antitumor effects on uterine cervix cell Hela and that at least in part.One of the important mechanisms of endostatin action on the potent antiangiogenic and antitumor activities may involve inducing apoptosis.更多还原