【摘要】 目的:研究Lewis肺癌移植鼠胸腺与脾脏CD4+ CD25+调节性T细胞数量及其相关基因Foxp3 mR-NA的表达特点,探讨CD4+ CD25+调节性T细胞在诱导肿瘤免疫耐受中的作用。方法:将传代培养的Lewis肺癌细胞接种于C57BL/6小鼠右腋皮下,建立Lewis肺癌模型。观测成瘤率、平均瘤重、肿瘤体积动态变化;采用MTT法检测胸腺及脾脏T淋巴细胞增殖功能;采用流式细胞术检测胸腺及脾脏CD4+ CD25+调节性T细胞数量变化;采用半定量RT-PCR方法检测肺癌小鼠胸腺与脾脏Foxp3 mRNA表达水平。结果:Lewis肺癌的移植成瘤率为100%,平均瘤重(3.34±1.79)g;胸腺T淋巴细胞增殖功能略有降低,但降低不显著(P>0.05),而脾脏T淋巴细胞增殖功能明显降低(P<0.05);胸腺及脾脏CD4+CD25+调节性T细胞数量均明显升高(P<0.05);Foxp3 mRNA在肺癌模型组的胸腺表达明显增高(P<0.05),在肺癌模型组脾脏Foxp3 mRNA表达中也略有增加,但增高不显著(P>0.05)。结论:CD4+CD25+调节性T细胞可能在肿瘤免疫耐受中发挥重要作用。更多还原

【Abstract】 Objective: To study the number of CD4+CD25+ Treg and the expression of Foxp3 mRNA in thymus and spleen in mice with Lewis lung cancer, and investigate the role of CD4+CD25+ Treg in tumor immunological tolerance. Methods: The models were established by injected subcutaneously to the right axilla of C57BL/6 mice with subculturing Lewis lung cancer cells. The rate of tumor forming, tumor weight, the dynamic change of tumor volume were observed. The changes of T lymphocyte proliferation in thymus and spleen were detected by MTT test .The changes of number of CD4+CD25+ Treg were detected by flow cytometer and the expression of Foxp3 in thymus and spleen were analyzed by semi-quantitative RT-PCR. Results: The rate of tumor forming was 100%, tumor weight was 3.34 1.79g. The changes of T lymphocyte proliferation in thymus was lower in the tumor mice than in the normal mice, but not significantly like in splenic lymphocytes(P>0.05). The changes of T lymphocyte proliferation in spleen was significantly lower in the tumor mice than in the normal mice(P<0.05). The number of CD4+CD25+ Treg in thymus and spleen of mice was markedly higher in the tumor mice than in the normal mice(P<0.05). The expression of Foxp3 mRNA in thymic lymphocyte was significantly higher in tumor mice (P<0.05).The expression of Foxp3 mRNA in splenic lymphocyte was also shown higher in tumor mice than normal mice, but not significantly like in thymic lymphocytes(P>0.05). Conclusion: CD4+CD25+regulatory T cell might be responsible for immune suppression in the tumor immunity and play it’s function in tumor tolerance.更多还原