【摘要】 目的:氨基胍和维生素C对自发糖尿病肾病大鼠基膜粘连蛋白基因表达的调节。方法:将实验用6月龄SPF级GK大鼠和Wistar大鼠分为正常对照组、糖尿病组、维生素C治疗组、氨基胍治疗组、维生素C和氨基胍联合治疗组。治疗16周。观察治疗期间及治疗后大鼠的一般状况、血糖、尿素氮、血肌苷,内生肌苷清除率、24h尿蛋白排泄率,原位杂交检测肾小球基膜粘连蛋白B1的基因表达。结果:1造模组大鼠均出现肾脏功能有损害2氨基胍和维生素C对血糖无影响,但能改善基本状况,降低糖尿病大鼠的尿素氮、血肌苷、24h尿白蛋白排泄率,增加内生肌苷清除率,基膜粘连蛋白B1基因表达显著下调。结论:氨基胍和维生素C无降糖作用,但可通过降低基膜粘连蛋白基因表达,有确切的肾脏保护作用。更多还原

【Abstract】 Objective: To observe the regulation of aminoguanidine and vitamin C on genic expression of laminin in spontaneous diabetic nephropathy rats .Methods: Six months pecefic pathogen free(SPF) GK rats and wistar rats were allocated to normal control group, model group, vitamin C group, Aminoguanidine group, Vitamin C and Aminoguanidine group at random. All rats were treated with corresponding drugs for 16 weeks. During and after the treatment , the general state, blood gloucose levels , blood urea nitrogen ,serum creatinine, urinary albumin excretion rate of the 24hours, and clearance rate of creatinine, as well as type laminin B1 mRNA were determined by in situ hybridization analysis in different groups. Results: ① Diabetes mellitus and renal function lesion occurred in the four model groups. ②Vitamin C and Aminoguanidine have no effect on the blood gloucose levels, while they could improve the general state, decrease blood urea nitrogen ,serum creatinine, and urinary albumin excretion rate of the 24hours; increase clearance rate of creatinine,The expression of laminininB1 mRNA in the kidney were significantly inhibited at the same time.Conclusion: Aminoguanidine and vitamin C have no effect in decreasing the blood sugar level but have a certain in preventing renal . by inhibiting gene expression of laminin.更多还原