【摘要】 目的建立月季花中总黄酮含量的测定方法。方法以芦丁为对照品,利用紫外分光光度法于510nm处测定月季花中总黄酮的含量。结果芦丁在5-50μg/ml范围内呈现良好的线性关系,回归方程为:Y=0.084A-0.0020,r=0.9996,总黄酮平均回收率为99.97%。月季花中总黄酮含量为0.930%,RSD=0.6%。结论比色法简便、快速、准确、灵敏,可作为测定月季花中总黄酮含量的方法。更多还原

【Abstract】 Objective To establish a method for determining the content of total flavone in Rosa chinensis Jacp. Methods The total flavonoid in Rosa chinensis Jacp was determined by ultraviolet spectrophotometry at 510 nm, with rutin as a reference substance. Results The standard curve was linear in the concentration range of 5-50 μg/ml.Linear regression equation was Y=0.084A-0.002 0,r=0.999 6.The average recovery of total flavones was 99.97%.The content of flavones was 0.930% in Rosa chinensis Jacp,RSD=0.6%. Conclusion The method is simple, rapid and accurate.It can be used for determining the content of total flavone in Rosa chinensis Jacp.更多还原