【摘要】 目的建立一种快速预检ELISA预包被板条的实验室方法。方法利用现代优质酶联免疫检测仪的基础酶联软件对预包被板条基础空白比色,并与以预包被板条模拟未包被板条批检的标准方法(参考方法)进行比较。结果模拟前后,总体方差齐(F检验P>0.05,a=0.05),相应的行预检与模拟A平均值的离均差与行模拟净显色A平均值的离均差均无显著性差异(配对计量资料比较t检验:P>0.05,a=0.05)。结论利用基础酶联软件直接预检预包被板条是简便实用的,具有评价预包被板条质量和选板设置数据复核的双重功效;试剂生产单位或厂家也可以对预包被板条二次批检。更多还原

【Abstract】 Objective To develop a method in clinical laboratories for rapid detection of ELISA pre-coating plates.Methods Basic blank absorbances of pre-coating plates were measured by primaryEIA of a contemporary and high quality ELISA analysis equipment,and contrast with the standard(reference)method of batch detection of no coating plates.Results Before and after simulating,analysis of variance(ANOV)was homogeneity of population(F-test:P>0.05,a=0.05),and correspondingdeviations of mean dispersion for basic blank and simulating mean absorbances of every row and every row mean absorbances of net reaction colour were both no significant defference(t-test:P>0.05,a=0.05).Conclusion Direct detection is handy and practical,is of double functions of evaluating the quality of ELISA pre-coating plates and setting up blank and controlor standard values positions and/or checking A values,and pre-coating plates are also checked a second in units and companies or enterprises of making ELISA reagent kits.更多还原