【摘要】 磷钼杂多酸与甲胎蛋白(AFP)结合,会引起共振瑞利散射(RRS),最大RRS峰均位于480 nm,在一定浓度范围内,AFP浓度与散射强度成正比。本文对该反应体系的适宜反应条件、主要影响因素、散射强度与AFP浓度的关系、方法的灵敏度等进行了比较研究。发现不同的杂多酸对于甲胎蛋白的检出限(3σ)在5.2-78 ng/mL之间,其中以磷锑钼酸体系灵敏度最高,对于甲胎蛋白(AFP)的检出限为2.5 ng/mL。该方法具有操作简便、灵敏度高、线性范围宽等优点。考察了共存物质的干扰影响,获得了满意的结果。更多还原

【Abstract】 When protein reacts with some large inorganic acid anions such as molybdate to form new products,a maximum resonance scattering(RS) peak at 480 nm for the system of protein-phosphomolybdic acid(PMA) was observed,the optimum conditions,effective factors,the relation between the scattering intensities and the concentration of protein,and the sensitivity of the method have been investigated.The detect limit of different protein-phosphomolybdic acid varies from 5.2 to 78 ng/mL.The sensitivity of molybdate-protein system is the highest,and the detect limit of AFP is 2.5 ng/mL.The method has advantages of easy operation,high sensitivity and wide determination range.Proteins in synthetic samples have been determined by the method with satisfactory results.更多还原