【摘要】 采集翅果油树花粉(乡宁种群17个样、翼城种群10个样)为实验材料,对贮藏在不同温度(室温20℃、4℃和-4℃)下翅果油树花粉的生活力进行了测定,并探索适合翅果油树花粉生活力测定的方法及条件.结果表明:(1)I-KI染色法适合于翅果油树花粉生活力的检验,而TTC染色法和醋酸洋红染色法不适合;(2)在室温20℃的条件下,乡宁和翼城翅果油树花粉萌发的最佳培养基不同,10%蔗糖+0.1%硼酸适合乡宁翅果油树花粉生活力的测定,而15%蔗糖+0.1%硼酸适合于翼城翅果油树花粉生活力测定;(3)在相同的条件下,乡宁的翅果油树花粉生活力较翼城的好.更多还原

【Abstract】 The samples of Elaeagnus mollis pollen were collected in the field,including 17 samples from Xiangning and 10 samples from Yicheng,Shanxi.E.mollis pollen vitality was studied with treatments of different temperatures(20 ℃,4 ℃and-4 ℃).The measuring method and condition adapting to measure E.mollis pollen vitality were investigated.The results indicated that:(1)The testing method of staining with I-KI adapted to test E.mollis pollen vitality,but the testing method of staining with TTC and acetum magentas did not.(2)At 20 ℃,the best culture medium for that of E.mollis pollen’ germination from Yicheng and Xiangning was different.Sucrose 10 % and boron acid 0.1 % adapted to test Xiangning’,and sucrose 15 %,boron acid 0.1 % adapted to test Yicheng’.(3)With the same condition E.mollis pollen vitality from Xiangning was better than Yicheng.更多还原